Proteome profiling of Populus euphratica Oliv. upon heat stress.
نویسندگان
چکیده
BACKGROUND AND AIMS Populus euphratica is a light-demanding species ecologically characterized as a pioneer. It grows in shelter belts along riversides, being part of the natural desert forest ecosystems in China and Middle Eastern countries. It is able to survive extreme temperatures, drought and salt stress, marking itself out as an important plant species to study the mechanisms responsible for survival of woody plants under heat stress. METHODS Heat effects were evaluated through electrolyte leakage on leaf discs, and LT(50) was determined to occur above 50 degrees C. Protein accumulation profiles of leaves from young plants submitted to 42/37 degrees C for 3 d in a phytotron were determined through 2D-PAGE, and a total of 45 % of up- and downregulated proteins were detected. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)/TOF analysis, combined with searches in different databases, enabled the identification of 82 % of the selected spots. KEY RESULTS Short-term upregulated proteins are related to membrane destabilization and cytoskeleton restructuring, sulfur assimilation, thiamine and hydrophobic amino acid biosynthesis, and protein stability. Long-term upregulated proteins are involved in redox homeostasis and photosynthesis. Late downregulated proteins are involved mainly in carbon metabolism. CONCLUSIONS Moderate heat response involves proteins related to lipid biogenesis, cytoskeleton structure, sulfate assimilation, thiamine and hydrophobic amino acid biosynthesis, and nuclear transport. Photostasis is achieved through carbon metabolism adjustment, a decrease of photosystem II (PSII) abundance and an increase of PSI contribution to photosynthetic linear electron flow. Thioredoxin h may have a special role in this process in P. euphratica upon moderate heat exposure.
منابع مشابه
Transcriptome Profiles of Populus euphratica upon Heat Shock stress
Heat stress, which strongly affects plant performance and often results in reduced vegetative growth and yields depression, has become an increasingly serious global problem. Populus euphratica Oliv. which has been considered as a tree model for the study of plant response to abiotic stresses, could be resistant to an extremely wide environmental temperature range (-40 °C to 45 °C). Previous st...
متن کاملEffects of NaCl on growth, yield and ion concentration of various Populus euphratica Oliv. ecotypes in Iran
Euphrates poplar (Populus euphratica Oliv.) is a woody species that is naturally distributed in the desert areas of some parts of Asia and Africa. Because of its outstanding features, it is a model plant to study environmental stress tolerance. This research was conducted from 2014 to 2016 in order to study the relationship between performance indices and ion concentrations. The cuttings ...
متن کاملGenome-wide characterization of new and drought stress responsive microRNAs in Populus euphratica
MicroRNAs (miRNAs) are small, non-coding RNAs that play essential roles in plant growth, development, and stress response. Populus euphratica is a typical abiotic stress-resistant woody species. This study presents an efficient method for genome-wide discovery of new drought stress responsive miRNAs in P. euphratica. High-throughput sequencing of P. euphratica leaves found 197 conserved miRNAs ...
متن کاملبررسی هتروزیس در دورگهای بین گونه‌ای در صنوبر
به منظور بررسی میزان هتروزیس در دو رگهای بین گونه‌ای تولید شده در صنوبر کبوده و پده (Populus euphratica OLIV و Populus alba L.), قلمه‌های تهیه شده از پایه‌های سه ساله دورگ و والدین آنها ابتدا در گلخانه ریشه‌دار شده و بعد در مزرعه در قالب طرح آماری بلوکهای کامل تصادفی غرس گردیدند. نتایج حاصل از داده‌ها بیانگر وجود اختلاف معنی‌داری بین والدین و نتاج برای قطرتنه و ارتفاع در دورگهای اصلی و متقابل م...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Annals of botany
دوره 98 2 شماره
صفحات -
تاریخ انتشار 2006